Pengaruh Suplementasi Isolat Protein Sorghum terhadap Sifat Kimia, Biologis dan Organoleptik Biskuit Sorghum
(Effect of Supplementation Isolate Sorghum Protein to Chemical, Biological and Organoleptic Properties of Biscuits Sorghum)
Pengaruh Suplementasi Isolat Protein Sorghum terhadap Sifat Kimia, Biologis dan Organoleptik Biskuit Sorghum
(Effect of Supplementation Isolate Sorghum Protein to Chemical, Biological and Organoleptic Properties of Biscuits Sorghum)
Fatim Illaningtyas
Teknologi Bioindustri BPPT, Jakarta, Indonesia
Sri Istini
Teknologi Bioindustri BPPT, Jakarta, Indonesia
Sri Peni W
Teknologi Bioindustri BPPT, Jakarta, Indonesia
Iim Sukarti
Teknologi Bioindustri BPPT, Jakarta, Indonesia
Fajarwati Utami
Teknologi Bioindustri BPPT, Jakarta, Indonesia
DOI:
ABSTRACT
The aims of the research were to determine the potential of isolate sorghum protein as ingredient functional food which focused on the effect of supplementation isolate sorghum protein to chemical, biological and organoleptic properties of biscuits sorghum. First phase of the application was made biscuit formula with a mixture of various wheat flour and sorghum flour (0: 100, 0:75, 0:50 and 0:25). The best formula biscuit from organoleptic test was added isolate sorghum protein 5, 10 and 15%, then the biscuit were analyzed of the chemical, biological and organoleptic properties. The results showed that the panelists preferred the formula biscuits with 25% sorghum flour. Chemical analysis showed that biscuit with sorghum isolate protein increase protein, amino acid, phenolic, and antioxidant activity. There were characteristic of biscuits with 15% isolate sorghum protein 3.8% fiber, 11.87% protein, 7.96% amino acid, 0.808 mg TAE/mg phenolic and 40.675 g/g BHA antioxidant activity. Suplementation of isolate shorgum protein increase the fiber, antioxidants and proteins content. Further more could be applied in to functional food products, such as food products for hypoglycemic, antioxidant, diabetes mellitus or for KEP patients.
Keywords: isolate sorghum protein, sorghum, biscuits, antioxidants, functional food
REFERENCES
Alu’datt, M.H., Taha Rababah, Khalil Ereifej, Inteaz Alli, Mohammad A. Alrababah, Ali Almajwal, Nather Masadeh and Mohammad N. Alhamad. 2011. Effects of Barley Flour And Barley Protein Isolate on Chemical, Functional, Nutritional and Biological Properties of Pita Bread. Journal online.
Awika, Joseph M., Lloyd W. Rooney. 2004. Sorghum Phytochemicals and Their Potencial Impact on Human Health. J. science Phytochemistry. Vol 65: 1199 – 1221.
Budijanto, S., A.B. Sitanggang dan W. Murdiati. 2011. Karakterisasi Sifat Fisiko Kimia dan Fungsional Isolate Protein Biji Kecipir (Psophocarphus tetragonolobus). J. Teknol. dan Industri Pangan. Vol 12 (2): 130-136
Bean, S.R., B.P. Ioerger, S.H. Park and H. Singh. 2006. Interaction Between Sorghum Protein Extraction and Precipitation Condition on Yield, Purity and Composition of Purified Protein Fraction. Cereal Chem. 83 (1): 99 – 107
Hartanto, H. 2012. “Identifikasi Potensi Antioksidan Minuman Cokelat dari Kakao Lindak (Theobroma Cacao L.) dengan Berbagai Cara Preparasi: Metode Radikal Bebas 1,1 Diphenyl-2-Picrylhydrazil (DPPH)”. Skripsi. Universitas Katolik Widya Mandala. Surabaya.
Henry. R. J and P. S. Kettlewell. 1996. In R. J. Henry and Kettlewell, Editors. Cereal Grain Quality.Chapman and Hall. London.
Kholis, N. dan F. Hadi. 2010. Pengujian Bioassay Biskuit Balita yang Disuplementasi Konsentrat Protein Daun Kelor (Moringa oleifera) pada Model Tikus Malnutrisi. Jurnal Teknologi Pertanian, 11(3): 144-151.
Kinsella, J. E. 1976. Functional Properties of Protein in Foods: a Survey. Critical Reviews in Food Science and Nutrition. 7 (1976): 219-280
Mimura, A. Protocols for Measurement of Antioxidative Activities. Departement of Applied Chemistry and Biotechnology, Faculty of Engineering, University of Yamanashi.
Napitupulu, A. 2006. “Kajian Pemanfaatan Tepung Sorghum dalam Pembuatan Biskuit Marie”. Skripsi. IPB. Bogor.
Rufaizah, Ummi. 2011. “Pemanfaatan Tepung Sorghum (Sorghum bicolor L moench) pada Pembuatan Snack Bar Tinggi Serat Pangan dan Sumber Zat Besi Untuk Remaja Puteri”. Skripsi. IPB. Bogor.
Salimi, Y.K. 2011. “Peranan Ekstrak dan Tepung Sorghum (Sorghum bicolor L-Moench) dalam Penghambatan Kanker secara in Vitro dan in Vivo pada Mencit BALB/c”. Thesis. IPB. Bogor.
Sasmito, E., Sri Mulyaningsih, Eka Kartika Untari dan Ratna Widyaningrum. 2006. Aktivitas Imunostimulan Susu Kedelai terhadap Imunoglobulin (IgG, IgA) dan Proliferasi Sellimfosit pada Mencit Balb/c yang Diinduksi Hepatitis A. Majalah Farmasi Indonesia, 17(3), 156– 161, 2006.
Sirappa, M. P. 2003. Prospek Pengembangan Sorgum sebagai Komoditas Alternatif Pangan, Pakan dan Industri. Jurnal Litbang Pertanian, 22(4).
Stonestreet, N. J. M, S. Alavi and S. R. Bean. 2010. Sorghum Proteins: The Concentration, Isolation, Modification and Food Applications of Kafirin. J. Food Science. 75(5): R90–R104
Supari, S.F. 2007. Spesifikasi Teknis Makanan Pendamping ASI (MP-ASI) Biskuit untuk Anak 12-24 Bulan. Keputusan Menkes No 224/Menkes/SK/II/2007, Jakarta.
Taylor, J. R. N., Schober, T. J., & Bean, S. R. 2006. Novel Food and Non-Food Uses for Sorghum and Millets. J. Cereal Science, 44(3): 252–271.
Yamaguchi, T., Takamura, T.,Terao, J.1998. HPLC Method for Evaluation of The Free Radical-Scavenging Activity of Foods by Using 1,1-Diphenyl-2-Picrylhydrazyl. Biosci. Biotechnol. Biochem. 62: 1201-1204
Yousif, A., D. Nhepera and S. Johnson. 2012. Influence of Sorghum Flour Addition on Flat Bread in Vitro Starch Digestibility, Antioxidant Aapacity and Consumer Acceptability. Food Chem. 134 (2012): 880 – 887.
Zakaria, F.R. 1996. Sintesis Senyawa Radikal dan Elektrofil dalam dan oleh Komponen Pangan. Dalam Prosiding Seminar dan Sistem Pangan, Reaksi Biomolekul, Dampak Terhadap Kesehatan dan Penangkalannya. Kerjasama Pusat Studi Pangan dan Gizi dengan Kedutaan Besar Prancis, Jakarta. Fransiska, RZ, Sedarnawati dan Ratih Dewantin (eds.) 4 April 1996, Bogor.
Published
20-06-2014
Issue
Vol. 8 No. 1 2014: Jurnal Agroteknologi
Pages
37-50
License
Copyright (c) 2014 Jurnal Agroteknologi
How to Cite
Illaningtyas, F., Istini, S., Peni W, S., Sukarti, I., & Utami, F. (2014). Pengaruh suplementasi isolat protein sorghum terhadap sifat kimia, biologis dan organoleptik biskuit sorghum. Jurnal Agroteknologi, 8(1), 37-50.