Aktivitas Antitumor dan Prebiotik Senyawa Turunan Epiglukan: (1→3),(1→6)-β-Glukan Ekstraselluler dari Epicoccum nigrum Ehrenb. Ex Schlecht
(Prebiotic and Antitumor Activities of Extracellular Epiglucan Produced by Epicoccum nigrum Ehrenb. Ex Schlecht)
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Jayus
Dosen Fakultas Teknologi Pertanian, Universitas Jember, Jember, Indonesia
Nuriman
Dosen Fakultas Keguruan dan Ilmu Pendidikan, Universitas Jember, Jember, Indonesia
Sony Suwasono
Dosen Fakultas Teknologi Pertanian, Universitas Jember, Jember, Indonesia
DOI:
ABSTRACT
Extracellular epiglucan produced by Epicoccum nigrum has been examined by in vitro analysis to show its potency as an antitumor substance against leucemia cell. The aims of this study were to ditermine the LC50 value and prebiotic activities of native and derivated epiglucan. The epiglucan was modified by sulfation and carboxymethilation methods. The result showed that LC50 of native epiglucan occur at concentration of 22 g/ml. This substance was chemically modified through sulfation and charboxymethilation process in order to increase its antitumor activity. Based on the value of LC50, the sulfated and carboxymethylated epiglucan have higher activity against leucemia cell compare to that of unmodified epiglucan. The LC50 value of sulfated epiglucan is 14 μg/L, while the carboxymethylated one is 13 μg/L. Prebiotic activities of both sulfated and carboxymethylated epiglucan were also higher compare to the unmodified glucan since these two derivatives are able to elevate the growth rate of probiotic microorganism such as Lactobacillus casei, L. acidophilus and L. rhamnosus.
Keywords: antitumor, extracellular epiglucan, Epicoccum nigrum, charboxymethilation, sulfation
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Published
20-06-2011
Issue
Vol. 5 No. 1 2011: Jurnal Agroteknologi
Pages
12-20
License
Copyright (c) 2011 Jurnal Agroteknologi
How to Cite
Jayus, Nuriman, & Suwasono, S. (2011). Aktivitas antitumor dan prebiotik senyawa turunan epiglukan: (1→3),(1→6)-ϐ-glukan ekstraselluler dari Epicoccum nigrum Ehrenb. Ex Schlecht. Jurnal Agroteknologi, 5(1), 12-20.
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