Aktivitas Antioksidan Hidrolisat Protein Ikan Wader (Rasbora jacobsoni) dari Hidrolisis oleh Enzim Calotropin dan Papain
(Antioxidant Activity of Protein Hydrolysates Common Barb Fish (Rasbora jacobsoni) from Hydrolysis by Calotropin and Papain Enzymes)
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Yuli Witono
Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Jember
Maryanto Maryanto
Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Jember
Iwan Taruna
Jurusan Teknik Pertanian, Fakultas Teknologi Pertanian, Universitas Jember
Ardiyan Dwi Masahid
Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Jember
Kinanti Cahyaningati
Jurusan Teknologi Hasil Pertanian, Fakultas Teknologi Pertanian, Universitas Jember
DOI: https://doi.org/10.19184/j-agt.v14i01.14817
ABSTRACT
The potency of common barb fish as fish protein hydrolyzate caused by its high protein content. Fish protein hydrolyzate is produced through the hydrolysis process by the calotropin enzyme and the papain enzyme. This study used common barb fish with one type of treatment, i.e. the difference combination of the calotropin (C) and papain (P) enzyme. Making fish protein hydrolyzate is used to determine the antioxidant activity of the common barb fish protein hydrolyzate. The observation parameters in this study were enzyme activity, antioxidant activity, degree of hydrolysis, molecular weight, amino acids, dissolved protein content, and water holding capacity. Samples with a ratio of 40C: 60P enzyme concentration are wader fish protein hydrolyzate with the proportion of 40% calotropin enzyme and 60% papain enzyme is the highest sample that has an antioxidant activity of 36.41%, reduced power value of 0.57, hydrolysis degree of 74.40% and total amino acids of 82.79%. Wader fish hydrolyzate in the 70C: 30P sample of 76.98% has a high water holding capacity because it can maintain the protein component in meat.
Keywords: antioxidant, calotropin, common barb fish, fish protein hydrolyzate, papain
REFERENCES
AOAC. 2005. Official methode analysis. association of official analytical chemists. Benjamin Franklin Station, Washington.
Amiza, M.A., Kong, Y.L., and Faazaz, A.L. 2012. Effect of hydrolysis on physicochemical properties of cobia (Rachycentron canadum) frame hydrolysate. Journal International Food Research, 19 (1): 199-206.
Badan Pusat Statistik Kabupaten Jember. 2013. Produksi dan nilai produksi budidaya perikanan air tawar menurut jenis produksi dan jenis perairan. (http://jemberkab.bps.go.id) [Diakses pada 29 Juli 2019].
Chalamaiah, M., Dinesh Kumar, B., Hemalatha, R., and Jyothirmaryl, T. 2012. Fish protein hydrolysates: proximate composition, amino acid composition, antioxidant activities and applications: A Review. Food Chemistry, 135: 3020-3038.
Charoenphun, N., Benjamas, C., Nualpun, S., and Wirote. 2013. Calcium-bindingpeptides derived from tilapia (Oreochromis niloticus) protein hydrolysate. European Food Research and Technology, 236(1): 57-63.
Dwiloka, B., dan Atmomarsono, U. 2007. Kandungan Logam Berat pada Daging Dada dan Paha Ayam Broiler yang Dipelihara dengan Sistem Kandang Panggung Setelah Direbus dan Dikukus. Staf Dosen pada Laboratorium Teknologi Hasil Ternak, Fakultas Peternakan, UNDIP. 235-242.
Gelichpour, M., and Shabanpour. 2011. The investigation of proximate composition and protein solubility in processed mullet fillet. International Food Research Journal, 18 (4): 1343-1347.
Ghassem, M., Fern, S.S., Said, M., Ali, Z.M., Ibrahim, S., and Babji, A.S. 2011. Kinetic characterization of channa striatus muscle sarcoplasmic and myofibrillar protein hydrolysates. Journal of Food Science and Technology. [Doi: 10.1007/ s13197-011-0526-6].
Haslaniza, H. 2010. The effects of enzyme concentration, temperature, and incubation time on nitrogen content and degree of hydrolysis of protein precipitate from cockle (Anadara granosa) meat wash water. International Food Research Journal, 17: 147-152.
Klompong, V., Benjakul, S., Yachai, M., Visessanguan, W., Shahidi, F., and Hayes, K.D. 2009. Amino acid composition and antioxidative peptides from protein hydrolysates of yellow stripe trevally (Selaroides leptolepis). Journal of Food Science, 74: C126-C133.
Kurniawan, S., Lestari., dan Hanggita, S.R.J. 2012. Hidrolisis protein tinta cumi-cumi (Loligo sp.) dengan enzim papain. Fishtech, 1 (1): 41-54.
Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of cateriophage T4. Journal Nature, 227: 680-685.
Mendis, E., Rajapakse, N., Byun, H.G., and Kim, S.K. 2005. Investigation of jumbo squid (Dosidicus gigas) skin gelatin peptide for their in vitro antioxidant effects. Journal of Life Science, 77: 2166-2178.
Murray, R. K., Granner, D. K., Mayes, P. A and Rodwell, V.W. 2003. Biokimia Harper. Penerbit Buku Kedokteran EGC, Ed 25, Jakarta.
Ovissipour, M., Benjakul, S., Safari, R., and Motamedzadegan, A. 2010. Fish protein hydrolysates production from yellowfin tuna thunnus albacares head using alcalase and protamex. International Aquatic Research, 2: 87-95.
Oyaizu, M. 1988. Antioxidative activity of browning products of glucosamine fractionated by organic solvent and thin layer cromatography. Journal Nippon Shokuhin Kogyo Gakkaisshi, Volume 35: 771-775
Poedjiadi A. 2006. Dasar-dasar Biokimia. UI-Press, Jakarta.
Purwanto. 2014. Evaluasi Hasil Belajar. Pustaka Pelajar, Yogyakarta.
Ranathunga, S., Rajapakse, N., and., Kim, S.K. 2006. Purification and characterization of antioxidative peptide derived from muscle of conger eel (Conger myriaster). Euro Food Res Technol, 222: 310–315.
Rao, M.B., Tanksale, A.M., Ghatge, M.S., and., Deshpande, V.V. 1998. Molecular and biotechnological aspect of microbial proteases. Microbiol Mol Biol Review, 62:597-635.
Shimada, K., Fujikawa, K., Yahars, K., and Nakamura, T. 1992. Antioksidative properties of xantan on the antioxidation of soy bean oil in eyelodextrin emultion. Journal of Agricultural and Food Chemistry, 40: 945-948.
Soeparno. 2009. Ilmu dan Teknologi Daging. Gadjah Mada University Press. Yogyakarta. 6; 152-156; 289-290; 297–299.
Tounkara, F., Bernard, S., Tidjani, A., Guo-Wei, L., and Yong-Hui Shi. 2013. Antioxidant effectand water-holding capacity of roselle (Hibiscus sabdariffa L.) Seed protein hydrolysates. Advance Journal of Food Science and Technology, 5 (6): 752-757.
Venugopal, V. 2006. Seafood Processing: Adding Value Through Quick Freezing, Retortable Packaging, and Cook-Chilling. CRC Pr, Boca Raton.
Walter, H.E., 1984. Method With Haemoglobin, Casein, and Azocoll as Substrate in. Bergmeyer. Hu (ed). Methods of Enzymatic Analysis. Verlag Chemie, Deerfield Beach Florida Basel.
Witono, Y., Aulanni’am, Subagio, A., dan Widjanarko, S.B. 2007. Preliminary Study for Enzymatic Processung of Milkfish Hydrolysate by Using “Biduri” Protease. Prosiding Seminar Nasional Perhimpunan Ahli Teknologi Pangan Indonsesia (PATPI), Bandung 17-18 Juli 2007.
Witono, Y. 2009. Spesifitas dan Stabilitas Protese Biduri (Calotropis gigantea). Tidak Diterbitkan. Prosiding Seminar Nasional Perhimpunan Ahli Teknologi Pangan (PATPI), Denpasar.
Witono, Y., Taruna I., Windrati. W.S., Azkiyah, L., and Sari, T.N. 2016. Wader (Rasboa jacobsoni) protein hydrolysates: Production, biochemical, and funcional properties. Agriculture and Agricultural Science Procedia, 9: 482-492.
Wu Hui-chun., Chen, H.M., and Shiau, C.Y. 2003. Free amino acids and peptides as related to antioxidant properties in protein hydrolysates of mackerel (Scomber austriasicus). Food Research, 36: 949-957.
Zayas, J.F. 1997. Funcionally of Protein in Food. Springer, Germany.
Published
20-06-2020
Issue
Vol. 14 No. 1 2020: Jurnal Agroteknologi
Pages
44-57
License
Copyright (c) 2020 Jurnal Agroteknologi
How to Cite
Witono, Y., Maryanto, M., Taruna, I., Masahid, A.D., & Cahyaningati, K. (2020). Aktivitas antioksidan hidrolisat protein ikan wader (Rasbora jacobsoni) dari hidrolisis oleh enzim calotropin dan papain. Jurnal Agroteknologi, 14(1), 44-57. https://doi.org/10.19184/j-agt.v14i01.14817
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